Cerebellar plasticity

In these papers we established highly sensitive non-radioactive in situ hybridization approaches for subcellular mRNA distribution and local translation studies. The work supports the Hebbian synapse concept of activity-dependent changes by showing electrical activity induced local translation in cerebellar Purkinje neurons.

Changing subcellular distribution and activity-dependent utilization of a dendritically localized mRNA in developing Purkinje cells.

Wanner I, Baader SL, Oberdick J, Schilling K.
Molecular and cellular neurosciences. 2000; 15(3):275-87.PubMed [journal]PMID:10736204

An optimized method for in situ hybridization with signal amplification that allows the detection of rare mRNAs.

Yang H, Wanner IB, Roper SD, Chaudhari N.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 1999; 47(4):431-46.PubMed [journal]PMID:10082745

Mapping of D1 dopamine receptor mRNA by non-radioactive in situ hybridization.

Lazarov NE, Schmidt U, Wanner I, Pilgrim C.
Histochemistry and cell biology. 1998; 109(3):271-9.PubMed [journal]PMID:9541476

Subcellular localization of specific mRNAs and their protein products in Purkinje cells by combined fluorescence in situ hybridization and immunocytochemistry.

Wanner I, Baader SL, Brich M, Oberdick J, Schilling K.
Histochemistry and cell biology. 1997; 108(4-5):345-57.PubMed [journal]PMID:9387927